Effect of Compound Danshen Dripping Pills on distribution of carbamazepine in brain of normal and refractory epilepsy rats / 中草药

ABSTRACT

Objective To investigate the effect of Compound Danshen (Salvia miltiorrhiza) Dripping Pills (CDDP) on the distribution of carbamazepine (CBZ) in the brain of normal and refractory epilepsy rats. Methods Wistar male rats were divided into normal and model rats. Normal rats were divided into control group and CDDP group. Model rats were divided into control group, CDDP group and verapamil group. The animal model of refractory epilepsy was induced by intrahippocampal injection of kainic acid. After the success of modeling, CDDP group was given CDDP (85 mg/kg), verapamil group was given verapamil (20 mg/kg) for 10 d, then each group was given CBZ (100 mg/kg) respectively. Brain tissue samples were collected at different time points. After the samples were pretreated, the concentration of CBZ in the brain tissue was detected by HPLC-MS/MS. Results When using carbamazepine alone, the concentration of CBZ in normal rats was obviously higher than epileptic rats. Compared with using carbamazepine alone, CDDP could significantly increase the concentration of CBZ in normal and epileptic rats. For the epileptic rats, the enhancing effect of CDDP to the cerebral distribution of CBZ was similar to that of P-glycoprotein inhibitor verapamil. Conclusion CDDP could enhance the permeability of blood-brain barrier to CBZ and increase the cerebral distribution of CBZ in normal and epileptic rats.