Determination of six components in ilex kudingcha with quantitative analysis of multi-components by single marker / 中草药

ABSTRACT

Objective To establish a quantitative analysis of multi-components by single marker (QAMS) for the simultaneous determination of six components in Ilex kudingcha by HPLC. Methods The purity of kaempferol-3-O-β-D-rutanoside, isorhamnetin-3-O-β-D-rutanoside, kudinoside C, kudinoside A, kudinoside E, and kudinoside D was determined by HPLC-MS in test sample taking kudinoside C as internal standard, relative correction factors of the other five compounds were determined, and their contents were calculated. At the same time, the contents of these six components were determined by external standard method (ESM), and the difference between ESM and QAMS was compared to verify the feasibility and accuracy of QAMS method. Results There was no significant difference of the six components’ contents calculated between QAMS method and ESM method. Conclusion It is convenient, feasible, and accurate to measure the content of six components in I. kudingcha by QASM method.