Study on HPLC fingerprint of Cocculus orbiculatus root and stem / 中草药

ABSTRACT

Objective: To evaluate the similarity of Cocculus orbiculatus root and stem by high performance liquid chromatography (HPLC) fingerprint, and to provide a reference for evaluation and clinical application of C. orbiculatus root and stem. Methods: HPLC was applied to chromatographic separation of C. orbiculatus root and stem in different seasons from 14 areas of Guizhou province. The chromatography conditions were Agilent ODS C18 (250 mm × 4.6 mm, 5 μm) column, mobile phase of CAN 0.1% H3PO4, running time of 95 min, flow speed of 1.0 mL/min, detection wavelength of 254 nm, and column temperature of 30 ℃. The data were processed by Chinese medicine chromatographic fingerprint similarity evaluation system 2.0. Different C. orbiculatus root and stem were subjected to similarity analysis. Results: The chromatography fingerprint of C. orbiculatus root and stem was established and eight common characteristic peaks were demonstrated. The similarities of the chromatography fingerprint were between 0.985-0.998. Conclusion: The chemical compositions of C. orbiculatus root and stem is similar, but the active substances quantity of C. orbiculatus from different regions and seasons are different.