Optimization of preparation process of ginsenosides Rh4 and Rk3 by response surface methodology / 中草药

ABSTRACT

Objective: To employ the response surface methodology (RSM) to optimize the preparation conditions for ginsenoside Rh4 (GRh4) and Rk3 (GRk3) using the yields as index. Methods: High temperature hydrolysis combined with RSM was used to prepare GRk3 and GRh4 from ginsenoside Rg1 (GRg1), and the reaction conditions were optimized at the same time. Results: The optimal preparation conditions of GRk3 and GRh4 were confirmed using Design Expert 7.1.6 software as followss acid concentration of 0.02%, reaction temperature of 116.4℃, and reaction time of 2.22 h, respectively. Under the above conditions, the yields of GRk3 and GRh4 were 123.56 mg with conversion rate of 61.78%. Conclusion: The preparation conditions obtained from RSM optimization are suitable for large scale preparation of GRk3 and GRh4.