Simultaneous determination of seven components in Chrysanthemum indicum by QAMS / 中草药

ABSTRACT

Objective: To establish a new method for the quantitative analysis of multi-components by single-marker (QAMS) to simultaneous determine seven components in Chrysanthemum indicum. Methods: The chlorogenic acid was used as internal marker to calculate the relative correlation factors (RCF) of caffeic acid, luteolin-7-O-glucoside, 3,5-O-dicaffeoylquinic acid, buddleoside, luteolin, and apigenin by high performance liquid chromatography (HPLC). The repeatability of RCF was investigated. The contents of seven components were determined by the external standard method and QAMS respectively. Results: The reproducibility of RCF was perfect. The value calculated by QAMS was consistent with the external standard method. Conclusion: The QAMS method for simultaneously measuring the content of seven components is feasible and accurate to evaluate the quality of Chrysanthemum indicum.