ISSR analysis on genetic diversity of Ferula syreitschikowii in Xinjiang province / 中草药

ABSTRACT

Objective: To study the genetic relationship and genetic diversity of Ferula syreitschikowii germplasm resources in Xinjiang province. Methods: A total of 96 F. syreitschikowii germplasm resources of Xinjiang province were used as experimental materials. Fifteen primers selected from 33 ISSR primers could obtain high polymorphism and reproducibility bands for ISSR molecular marker analysis. Results: Total by 292 DNA bands were amplified including 289 polymarphic bands, counting for 99.01%. Genetic similarity analysis showed F. syreitschikowii germplasm resources of Xinjiang province with high genetic diversity. Through the statistical software analysis, the average number of effective alleles was 1.725 6, the average Nei's genetic diversity index was 0.4048, the average Shannon's information index was 0.587 9, and the genetic similarity coefficients among tested clones ranged from 0.500 0 to 0.828 7. These 96 germplasm resources were divided into two groups and 13 subgroups by UPGMA analysis, which could distinguish the germplasm resources from different sources. Conclusion: From the molecular level, it reveals the relationship between the geographical distribution of F. syreitschikowii and the germplasm resources and higher genetic diversity of germplasm resources, which provids basis for identification of F. syreitschikowii varieties.