Study on HPLC digital fingerprint of Paeonia suffruticosa and determination of eight components / 中草药

ABSTRACT

Objective: To establish an HPLC digital fingerprint for flower petals of Danfeng (Paeonia ostii), and apply it in the determination of other six varieties of P. suffruticosa produced in Heze areas, while determining the contents of eight components in the flower petals. Methods: Nano-Micro C18 (250 mm × 4.6 mm, 5 μm) column was adopted and the gradient mobile phase consisted of acetonitrile (A)-0.1% formic acid (B) with a flow rate of 1.0 mL/min, the detective wavelength was 270 nm, and the column temperature was 25 ℃. Results: The chromatographic fingerprint similarity evaluation system for traditional Chinese medicines was used for analysis. The chromatographic fingerprint similarity of six batches of Danfeng samples was more than 0.993. There were altogether 14 common peaks, and eight of them were identified. A comparative analysis showed a similarity between samples of Danfeng and other six varieties produced in Heze. Meanwhile, the content of eight major chemical constituents, namely gallic acid, methyl gallate, kaempferol-3-O-β-D-glucopyranoside, apigenin-7-O-β-D-glucopyranoside, apigenin-7-O-β-D-neospheroside, dihydrokaempferol-7-O-β-D-glucopyranoside, paeoniflorin, and oxypaeoniflorin were determined in the range of 0.05-1.41, 1.01-4.41, 0.70-5.55, 4.60-18.30, 10.05-33.87, 0.09-1.76, 3.16-11.12, 0.85-2.02 mg/g. Conclusion: The combination of HPLC digital fingerprint and content determination could reflect its inherent quality in an all-round way, which provided scientific basis for the quality evaluation of P. suffruticosa.