Preparation and characterization of quercetin molecularly imprinted polymer microspheres / 中草药

ABSTRACT

Objective: To prepare quercetin molecularly imprinted polymers (MIPs) by molecular imprinting technique. Methods: This experiment used quercetin as template molecule, acrylamide (AM) as functional monomers, ethylene glycol dimethacrylate (EGDMA) as cross linking agent, and azodiisobutyronitrile (AIBN) as initiator. Using anhydrous ethanol and tetrahydrofuran as pore forming agent, quercetin MIPs were prepared by precipitation polymerization and mass polymerization methods; The optimal proportion between template and functional monomer was chosen by UV and IR; The microstructure of MIPs was investigated by SEM. The adsorption equilibrium time and maximum adsorption of MIPs prepared with two methods were investigated by balance and isothermal adsorption experiment, and the specific adsorption capacity was studied. Results: The experimental research showed that precipitation polymerization method of quercetin MIPs had uniform rules of globular structures. The adsorption kinetics and isothermal adsorption experiment found faster adsorption rate and larger adsorption capacity. Polymer on quercetin had high specificity recognition ability in rutin and quercetin adsorption selective process. Conclusion: Precipitation polymerization of MIPs, with strong selective adsorption, separation and enrichment of chemical components in Chinese medicine flavonoids complex, is a new method of research. At the same time, it also provides reference for chemical composition research of other Chinese materia medica.