Effect of Maxing Shigan Decoction against type A influenza virus infection in mice induced by viral lung injury based on TLR4-MyD88-TRAF6 signal pathways / 中草药

ABSTRACT

Objective: To explore the effect of TLR4 on type A influenza virus induced lung tissue injury, and to further explore the intervention effect of Maxing Shigan Decoction (MXSGD). Methods: The WT influenza virus model in mice infected with influenza A virus was established. The mice were devided into low, medium, and high dose MXSGD groups, oseltamivir group, IAV group, and Sham group. After 7 d, the treated animals were treated with corresponding clinical equivalent dose of drug. Detection in mouse body weight, lung index, spleen index, thymus index, pathological changes of lung, and ELISA method for the detection of alveolar irrigation lotion in inflammatory cytokines (TNF-α, IL-1β, and IL-6) content, real-time PCR and Western blotting detection of lung tissue TLR4, MyD88, TRAF6 mRNA, and protein expression. Results: MXSGD could up-regulate the weight, spleen index, and thymus index of type A influenza virus infection in WT mice, improve the pathological injury of lung tissue, decrease alveolar lavage lotion proinflammatory cytokines content, and down regulate the expression of MyD88 and TRAF6 mRNA and protein in lung index and lung tissue and there is a certain dose effect relationship. The effect of high dose MXSGD group was close to oseltamivir. Conclusion: MXSGD as effective anti influenza virus of traditional Chinese medicine compound can effectively reduce lung inflammation, protect immune organs, and regulate cytokine balance. The possible mechanism is alleviating the lung injury caused by type A influenza virus infection in mice through inhibition TLR4-MyD88-TRAF6 signaling pathway of activation.