Effect of Huoxue Dingxuan Capsule on gene expression profile in mice brain microvascular endothelial cell bEnd.3 and analysis in bioinformatics / 中草药

ABSTRACT

Objective: To investigate the effect of Huoxue Dingxuan Capsule (HDC) containing serum on gene expression profile in mice brain microvascular endothelial cell bEnd.3 and its molecular mechanism. Methods: Thirty rats were randomly divided into control group and HDC group, each group had 15 rats, and the serum was collected in two groups of rats. BEnd.3 was divided into blank serum group, hypoxia model group, and 10% HDC containing serum group. The cells were intervened in different conditions, and after oxygen deprivation for 6 h, the cytoskeleton was observed under laser microscope. And to detect the effect of HDC containing serum on gene expression profile in mice brain microvascular endothelial cell bEnd.3 by Affymetrix U133 plus2.0 gene expression microarray, and for clustering analysis by molecular annotation system MAS3.0. Results: Differentially expressed genes were identified based on P 3. There were 405 differentially expressed genes between blank group and model group, in which 176 genes were up-regulated and 229 genes were down-regulated. There were 368 differentially expressed genes between HDC sero group and model group, in which 146 genes were up-regulated and 222 genes were down-regulated. These differentially expressed genes had the functions of positive regulation of endothelial cell migration, process of acid metabolism, production of vascular endothelial growth factors, positive regulate activity of NF-κB transcription factors, oxidative stress-induced senescence, mitosis prophase, platelet aggregation (blockage formation), ect. And many signaling pathways were regulated by these genes including vascular endothelial growth factor signaling pathway, interleukin signal pathway, p53 pathway, TNF-signal pathway, PPAR signaling pathway, PI3K-Akt signaling pathway, apoptosis signal pathway etc. Conclusion: HDC has significant inhibitory effects on damage of bEnd.3 cells induced by hypoxia. Its mechanism is related to oxidative stress, inhibition of apoptosis, promotion of angiogenesis, inflammation, and immune response. Huoxue Dingxuan Capsule can regulate the function of vascular endothelial cell on gene level by several signaling pathways.