Isolation of endophytic fungi from Dendrobium officinale and preliminary evaluation of in vitro biological activity / 中草药

ABSTRACT

Objective: To study the distribution of endophytic fungi from Dendrobium officinale and screen the distinctive fungi with antimicrobial and antitumor activities. Methods: Endophytic fungi were isolated by the conventional method and cylinder-plate method with Staphylococcus aureus, Escherichia coli, and Bacillus subtilis as subjects. The metabolites of ethyl acetate extracts with antitumor activity were identified based on MTT assay. Results: A total of 28 strains of the endophytic fungi were isolated from D. officinale. By analyzing the data of molecular identity, there were 10 species in D. officinale, in which Fusarium Link is a dominant. The ethyl acetate extracts of the fermentation broth had different antibiotic activities on the indicator microorganisms, and had antitumor effect on HepG2 cells. Conclusion: Endophytic fungi isolated from D. officinale have rich diversity and have antimicrobial and antitumor activities, which could be potential for developing antimicrobial and antitumor reagents.