Determination of curcumin, desmethoxycurumin, and bisdesmethoxycurum in Curcuma longa by QAMS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 573-577, 2017.
Artigo
em Chinês
| WPRIM
| ID: wpr-853015
ABSTRACT
Objective:
To establish a quality evaluation method for Curcuma longa, quantitative of multi-component with a single-marker (QAMS), to determine the contents of three curcuminoids at the same time.Methods:
An HPLC method was used, and a Chromstar™ C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was methanol-4% glacial acetic acid (4852) at a flow of 1.0 mL/min. The detection wavelength was 422 nm and column temperature was 30℃. Curcumin was used as the internal reference substance. The relative correlation factors of curcumin, desmethoxycurumin, and bisdesmethoxycurumin to curcumin were calculated and evaluated by standard curve method and QAMS.Results:
The QAMS method could be used to determine the contents of three curcuminoids at the same time, and with no significant difference between the results of standard curve method (RSD < 2.0%).Conclusion:
QAMS method is simple, feasible, and reproducible, which could provide a reference for further research on completing the quality control of raw material and processed C. longa.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2017
Tipo de documento:
Artigo
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