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Study on blood-brain distribution of lactones in rats after oral administration of Ginkgo biloba extract by blood-brain synchronization microdialysis / 中草药
Chinese Traditional and Herbal Drugs ; (24): 272-277, 2017.
Artigo em Chinês | WPRIM | ID: wpr-853029
ABSTRACT

Objective:

To study the blood and brain drug concentration and drug distribution coefficient of lactones in rats after oral administration of Ginkgo biloba extract (GBE).

Methods:

Blood-brain synchronization microdialysis was used in combination with HPLC-MS/MS inthis study. Probes were buried into rat brain and jugular vein to collect the blood and brain micro dialysis liquid in rats after oral GBE in different periods. The lactones in the dialysate were analyzed by HPLC-MS/MS, and the blood and brain drug concentration was depicted according to the results. Then the drug distribution coefficients of each component in blood and brain (AUCbrain/AUCblood) were calculated.

Results:

After GBE suspension (600 mg/kg) oral gavaging in the rats, the blood samples and brain samples were obtained and detected by HPLC/MS/MS. Ginkgolides A, B, and C were detected in both blood and in brain, but bilobalide could be detected just in blood. The drug distribution coefficients in the blood and brain (AUCbrain/AUCblood) were as follows Ginkgolide A was 2.911%, ginkgolide B was 3.126%, and ginkgolide C was 0.337%.

Conclusion:

Simultaneous multiple microdialysis technique can be used to detect multiple components in different tissues simultaneously. It also has other characteristics, such as continuous sampling on living animals with small sample volume and little tissue damage, which can save animals. In all, simultaneous multiple microdialysis could be a good method for exploring the effect components in Chinese medicine and their distribution at effect sides.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional and Herbal Drugs Ano de publicação: 2017 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional and Herbal Drugs Ano de publicação: 2017 Tipo de documento: Artigo