Grey relational analysis of relationship between limonin compounds accumulation and functional genes expression of Citri Reticulatae Semen / 中草药

ABSTRACT

Objective: To study the biosynthesis mechanism and transshipment law of limonin compounds in Citri Reticulatae Semen, traditional medicinal variety Citrus reticulata "Dahongpao". Methods: In the process of seed growth and development, the contents of limonin, nomilin, and obacunone in stem, vane, peel, and seeds were determined by UPLC tangerine; Using grey relational analysis method, the correlation analysis between the contents and the cloning of squalene synthase gene (ss), squalene epoxidase gene (se), and glucose transferase (lgt) expression was performed. Results: Limonin compounds in different organs had the largest accumulation in seeds; The accumulation of limonin compounds in different organs was significantly correlated with ss, se, and lgt gene expression, but in the seeds the accumulation of limonin has the most closely correlation with ss, se, and lgt gene expression; The ss gene expression had the largest contribution on the accumulation of limonin, nomilin, and obacunone. Conclusion: The study provides reliable and scientific envidendce for the synthesis mechanism and transshipment law of limonin compounds.