Correlation between chemical constituents of Isatidis Radix and bioactivity of neuraminidase on anti-influenza virus / 中草药

ABSTRACT

Objective: To explore the relationship between chemical constituents of Isatidis Radix (IR)and the inhibition of bioactivity of neuraminidase (NA)on influenza virus in vitro. Methods: To establish the chemical fingerprints of IR extraction from different producing areas and determine the contents of common peaks by UPLC. To determine the activity of NA on influenza virus tested with IR by Kit. To analyze the correlation of the chemical information and biological effect by mathematical statistics methods such as grey correlation analysis and one-factor analysis. Results: The chemical contents and biological effect detection varied markedly in the products from different regions and by extracting methods. The result of comprehensive analysis showed that uridine and (R, S)-goitrin of common components in UPLC-fingerprints related with the inhibition of bioactivity of NA on influenza virus, and the content of (R, S)-goitrin was correlated significantly to the activity of NA. Conclusion: Based on the biological assay of NA on influenza virus, the correlation of the content of chemical components and biological effect is verified preliminarily, with a view to provide a more reliable data and reference for the selection and extraction of antiviral active ingredients fromIR.