Establishment of a method for determination of triterpenic acids in Jujubae Fructus based on quantitative analysis of multi-components by single-marker / 中草药

ABSTRACT

Objective: To develop a method for the simultaneous determination of several triterpenic acids in Jujubae Fructus and provide the technical support for the quality control of Jujubae Fructus. Methods: Betulinic acid was used as the internal reference substance, and the relative correlation factors (RCF) of oleanolic acid, ursolic acid, betulonic acid, oleanonic acid, and ursonic acid were determined by HPLC-ELSD. The contents of the five components were calculated according to the RCF, respectively. The contents of these six triterpene acids in 15 batches of Jujubae Fructus were determined by the external standard method. The rationality, feasibility, and repeatability for the method of quantitative analysis of multi-components by single-marker (QAMS) were verified by comparing with the results obtained from the external standard method. Results: The reproducibility of the RCF was acceptable. For the six triterpene acids, there was no significant difference between the quantitative results with the two different methods in the 15 batches of Jujubae Fructus. Conclusion: The method established in this research is accurate and feasible that it just needs to assay single-marker (betulinic acid) for the determination of six triterpenic acids in Jujubae Fructus simultaneously. Therefore, this method is helpful for the quality control of Jujubae Fructus and could provide a new reference for the quality assess of multi components in Chinese materia medica.