Regulation of honeysuckle HQT gene to chlorogenic acid biosynthesis in eukaryotic plant cells of Lonicerae Flos / 中草药

ABSTRACT

Objective: To clarify how the HQT gene regulates and controls the chlorogenic acid in eukaryotic cells of Lonicerae Flos by establishment of HQT gene eukaryotic expression system, construction and optimization of genetically modified tissue culture system. Methods: Using gateway cloning system to build HQT gene eukaryotic expression system, we genetically created engineered bacteria containing honeysuckle gene function HQT by Agrobacterium tumefaciens-mediated method. The successful transfection explants of callus induction were established and the HQT gene relative transcript level was amplified by semi-quantitative RT-PCR; The HPLC was used to determine the sample's content of chlorogenic acid, then the correlation between HQT gene polymorphism and content of chlorogenic acid was analyzed. Results: The content of chlorogenic acid in Lonicerae Flos callus quantity rises as its HQT gene expression. Conclusion: Honeysuckle HQT gene could regulate chlorogenic acid biosynthesis in eukaryotic cells.