Rapid analysis on chemical constituents from roots of Gentiana crasicaulis by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry / 中草药

ABSTRACT

Objective: An ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC- hybrid quadrupole-orbitrap MS) method was developed to rapidly analyze and identify the chemical constituents from the roots of Gentiana crasicaulis. Methods: The 100% methanol extract was separated on an ACQUITY UPLC BEH C18 column (100 mm ×, 2.1 mm, 1.7 μm), and eluted with a gradient of methanol-water containing 0.1% formic acid. Constituents in the roots of G. crasicaulis were identified by HRMS in the positive and negative ion modes using both full scan and two-stage threshold-triggered mass modes. Results: A total of 34 compounds, including 13 iridoids, 5 flavonoids, 5 esters, 3 triterpenoids, 2 organic acids, 1 phenylpropanoid, and 5 others compounds were identified. Among them, 12 compounds were unambiguously identified by comparing with reference standards. Fifteen compositions wre reported for the first time in the roots of G. crasicaulis. Conclusion: Chemical constituents in the roots of G. crasicaulis were identified fast with UPLC-Q-Exactive hybrid quadrupole-orbitrap mass spectrometer technology to provide the scientific basis for the study on the material basis of the separation and crassicaulis chemical constitents.