Determination of four triterpenoid acids from Guizhi Fuling Capsules by HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 3215-3218, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-853276
ABSTRACT
Objective:
To establish an HPLC method for the determination of four triterpene constituents (dehydrotumulosic acid, polyporenic acid C, 3-epi-dehydropachymic acid, and dehydropachymic acid) in Guizhi Fuling Capsules (GFC).Methods:
Chromatography conditions were Diamonsic C18 column (250 mm × 4.6 mm, 5 μm), system mobile phase was composed of acetonitrile (A)-0.2% HCOOH aqueous solution (C) in a linear gradient elution mode (0-70 min 50%-85% A; 70-80 min 85%-100% A; 80-90 min 100% A), detective wavelength was set at 242 nm, and column temperature was 40℃.Results:
The calibration curve was linear within 0.408-2.04 μg/mL (r = 0.999 9), 0.192-0.96 μg/mL (r = 0.999 5), 0.078-0.39 μg/mL (r = 0.999 5), and 0.075 6-0.378 μg/mL (r = 0.999 5) for dehydrotumulosic acid, polyporenic acid C, 3-epi-dehydropachymic acid, and dehydropachymic acid, respectively. The average recoveries were 97.5% (RSD = 1.4%, n = 5), 98.5% (RSD = 1.6%, n = 5), 97.2% (RSD = 1.2%, n = 5), and 102.3% (RSD = 1.8%, n = 5). Six batches of GFC sample were determined, The average contents of dehydrotumulosic acid, polyporenic acid C, 3-epi-dehydropachymic acid, and dehydropachymic acid were 0.070, 0.015, 0.030, and 0.061 mg/capsule, separately.Conclusion:
The method is simple, accurate, and can be used as a quality control method for GFC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2016
Tipo de documento:
Artigo
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