Multiple shoot culture and effective material production of Anoectochilus roxburghii / 中草药

ABSTRACT

Objective: To provide a new material for exploring the products of Anoectochilus roxburghii, and study the effect of explant lengths, BA concentration and light intensities on multiple shoot biomass and effective material accumulation of A. roxburghii; This paper also explored the feasibility of the bioreactor culture on the rapid proliferation of A. roxburghii. Methods: The optimum explant lengths for multiple shoot proliferation were selected, the effect of different BA concentration and light intensities on multiple shoot biomass and effective material accumulation were investigated. Additionally, the comparison was carried out between bioreactor culture and conventional solid culture for multiple shoot biomass and effective material accumulation of A. roxburghii. Results: Explants (5 mm) promoted multiple shoot proliferation, mutiple shoot biomass was significantly higher than explants (1 mm). MS medium supplemented with 2.5 mg/L BA was optimal for multiple shoot biomass accumulation but the maximum productivities of polysaccharide, flavonoid, and total phenolic were found at 2.0 mg/L BA. Multiple shoot biomass and effective material accumulation were more suitable under light condition than under dark condition. The biomass and production of polysaccharide, flavonoid, and total phenolic (678.3, 12.2, and 17.8 mg/L) of multiple shoot at 5 g/L of inoculation density were significantly better than in solid culture. Conclusion: Multiple shoot biomass and productivities of polysaccharide, flavonoid, and phenolic compound are enhanced when 5 mm explants are inoculated in MS medium supplemented with 2.0 mg/L BA and cultured under light condition. In addition, bioreactors are used for large scale propagation of multiple shoot in less time. The accumulation of biomass and bioactive compound during bioreactor culture is higher than in solid culture.