HPLC determination of notoginsenoside Fc, a hemostatic and activating blood component, in different parts of Panax notoginseng / 中草药

ABSTRACT

Objective: To determine the contents of notoginsenoside Fc in the stems, leaves, flowers, fruits, fruit stalks, and roots of Panax notoginseng in Wenshan, and provide the scientific basis for the selection of raw materials and preparation. Methods: Using chromatographic column Agilent C18 (250 mm × 4.6 mm, 5 μm); mobile phase was CH3OH-H2O (65:35); Column temperature was 35℃; Flow rate was 1.0 mL/min; UV detection wavelength was 203 nm; injection volume was 10 μL. Using methanol as the solvent and ultrasonic extraction to extract the medicinal materials, then using the HPLC-UV method to determine the contents of notoginsenoside Fc in different parts of P. notoginseng. Results: Fc had a good linear relationship when the concentration was 0.005-1.000 mg/mL. The Fc contents in the cauline, leaves, flowers, fruits, fruit stalks, and roots of P. notoginseng were 0.066%, 1.58%, 2.13%, 0.39%, 0.72%, and 0.019%. The recovery rates were 98.86% (RSD = 1.90%), 97.43% (RSD = 1.87%), 101.03% (RSD = 1.80%), 99.35% (RSD = 1.82%), 99.13% (RSD = 1.94%), and 98.68% (RSD = 1.88%). Under this condition, Fc was not detected in the basal part of stems of P. notoginseng. Conclusion: Fc exists in different parts of P. notoginseng in Wenshan, and the contents of notoginsenoside Fc in the flowers and leaves of P. notoginseng were higher, 1.58% and 2.13%, respectively. The results can provide a scientific reference for the comprehensive utilization of the leaves and flowers of P. notoginseng and the preparation and application of Fc.