Prescription of triptolide nanometer liposomes and its preparation technology / 中草药

ABSTRACT

Objective: To optimize the preparation method of triptolide-nano-liposomes (TP-NLS). Methods High pressure homogeneous method was used to prepare TP-SLN. According to even design U7(73), the preparation method of TP-SLN was optimized with the factors including weight ratio of phosphorlipid and cholesterol (A), quantity of Poloxamer 188 (B), and homogeneous pressure (C), using the encapsulation efficiency (EE), particle size, and Zeta potential of NLS as indexes. Results: The optimum prescription of TP-NLS was A1B5C7, i.g. lipid matrix a b was 6 1, the dosage of Poloxamer 188 was 1.3 g, and the homogeneous pressure was 70 MPa, high pressure homogeneous method for 15 min. The TP-NLS prepared with the optimal method had good appearance. The EE was 83.52%, the average particle size was 117 nm, and the Zeta potential was 31.7 mV. TP-NLS solution was kept in avoiding light environment at 4℃ for 30 d, and the preservation stability was good. Conclusion: The formula is reasonable and the preparation method of TP-NLS is feasible, which is valuable to further study.