Analysis on chemical constituents from Forsythiae Fructus by HPLC-Q-TOF-MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 2053-2060, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-853451
ABSTRACT
Objective:
To analysis the main chemical ingredients of Forsythiae Fructus by LC-MS technology in the positive and negative ions mode.Methods:
The chromatographic fingerprint was obtained with Diamonsil II C18 column (250 mm×4.6 mm, 5 μm) and gradient elution with 0.05% H2O-formic acid (A)-acetonitrile (B), and the flow rate was 1.0 mL/min. The column temperature was maintained at 35℃. The detection wavelength was 200-600 nm. Positive and negative ions MS information of Bruker Daltonics 1200 HPLC-Q-TOF was coupled with elemental analysis and compared with literature data to analyze the compounds information.Results:
Combined with the accurately relative molecular mass of compounds provided by HPLC-Q-TOF-MS, 24 compounds were identified from the ethanolic extraction of Forsythia Fructus, which include 12 phenylethanoid glycosides, seven lignan ingredients, and five flavonoids. And 3,4-dihydroxyphenylethanoid glycoside (1), 2-methoxy-3,4,5-trihydroxyphenylethanoid glycoside (2), β-hydroxyforsythoside H (7), dihydroquercetagetin (24), and dihydromyricetin (25) were first identified in Forsythiae fructus.Conclusion:
The method provides the technical support for the quality control of Forsythiae Fructus and contributes the reference data to elucidating the potential basis of Forsythiae Fructus.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2016
Tipo de documento:
Artigo
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