Study on amelioration of Dendrobium chrysotoxum on STZ-induced non-proliferative diabetic retinopathy and its engaged mechanism / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1908-1913, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-853491
ABSTRACT
Objective:
To observe the amelioration of ethanol extract from Dendrobium chrysotoxum (EEDC) on non-proliferative diabetic retinopathy (NPDR) induced by streptozotocin (STZ), and further to investigate its engaged mechanism.Methods:
NPDR was induced by STZ injection in C57 mice and then the diabetic mice were orally given EEDC. The retinal blood-retinal barrier (BRB) breakdown was evaluated using Evans blue leakage assay. The mRNA expression of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF) α, early growth response-1 (Egr-1), tissue factor (TF), and Serpine 1 in retinas was detected by real-time PCR. The amount of IL-1β, IL-6, TNF-α, TF, and Serpine 1 in serum was detected by enzyme-linked immunosorbent assay (ELISA).Results:
EEDC at both doses reduced the increased Evans blue leakage in STZ-induced NPDR in mice.Results:
of Real-Time PCR showed that EEDC reduced the increased retinal mRNA expression of IL-1β, IL-6, TNFα and Egr-1, TF, Serpine 1 in STZ-induced NPDR in mice. ELISA results also confirmed that EEDC reduced the increased serum levels of IL-1β, IL-6, TNF-α, TF, and Serpine 1 in STZ-induced NPDR in mice.Conclusion:
EEDC could ameliorate the STZ-induced NPDR in mice via inhibiting retinal BRB breakdown by reducing the expression of pro-inflammatory cytokines including IL-1β, IL-6, TNF-α, and coagulation-fibrinolysis related signals such as TF and Serpine 1.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2016
Tipo de documento:
Artigo
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