Optimization of processing technology for cream with mixed accessories of oxhide gelatin and honey by central composite design-response surface methodology / 中草药

ABSTRACT

Objective: To clarify the optimum conditions for the preparation of mixed materials of oxhide gelatin and honey. Methods: Using the dosage of oxhide gelatin and honey as well as the relative density of refined cream as factors, and the paste forming and fingerprints as control indexes, the central composite design and response surface method was used to optimize the paste preparation process. Results: The extraction process was as follows: 6 times of water amount, soaked for 0.5 h, extracted for three times and each time was 1 h, which was 8 times of water for the first time. The main chromatographic conditions of paste fingerprints were as follows: Mobile phase was acetonitrile-0.1% phosphoric acid gradient [acetonitrile: 10% (0-5 min); 10%-35% (5-15 min); 35%-85% (15-30 min)], and detection wavelengths were 238 and 280 nm. Paste fingerprint: 12 common peaks were found, and the peak No. 7 was identified on honokiol. The RSD values of precision, reproducibility, and stability of each peak relative retention time and relative peak area precision were all less than 3%. The best conditions in the preparation process of cream were as follows: oxhide gelatin (8%-12%), honey (22%-28%), the refined cream and relative density (1.20-1.25). The absolute value of the deviation was less than 3% of paste preparation amplification experiments. Conclusion: This method can be used to ensure that the paste had good formability, and the chemical composition is relatively high.