Determination of 20(R)-25-OH-PPT in different parts of species in Panax Linn. by HPLC-ELSD methods / 中草药

ABSTRACT

Objective: For making full use of our country's rich ginseng resources to look for 25-OH-PPT\ and provide the scientific basis for the innovation and development of ginsenoside hypoglycemic activity. Methods: Using chromatographic column Agilent C18 (250 mm × 4.6 mm, 5 μm); mobile phase was CH3OH-H2O (7030); Column temperature was 35 ℃; Flow rate was 1.0 mL/min; the boiling chamber temperature of ELSD was 87 ℃; Flow rate of carrier gas was 2.20 L/min; injection volume was 10 μL. Using acetone as the solvent and ultrasonic extraction to extract the medicinal materials, then using the HPLC-ELSD method to determine the contents of 20(R)-25-OH-PPT in different parts of plants in Panax ginseng. Results: 20(R)-25-OH-PPT had a good linear relationship when the concentration was 0.001-0.05 mg/mL. The 20(R)-25-OH-PPT contents in gingseng cauline leaf, gingseng root, gingseng fruit, gingseng flower, cauline leaf of American ginseng, root of American ginseng, and the basal part of stem of P. notoginseng were 3.74%, 2.59%, 0.40%, 2.37%, 0.68%, 0.22%, and 0.39%. The recovery rates were 98.54%, 97.54%, 100.35%, 101.46%, 97.46%, 99.14%, and 99.38%. Conclusion:25(R)-OH-PPT exists in different parts of P. ginseng, and it can be an important basis for the use and development of ginsenoside with hypoglycemic activity.