Mechanisms of tonifying blood with Lvjiao Buxue Granules studied by1H-NMR metabolomics / 中草药

ABSTRACT

Objective: To explore the change of endogenous metabolites in serum of rats with erythronoclastic anemia and the intervention of Lvjiao Buxue Granules by using 1H-NMR metabonomics coupled with multivariate statistical analysis. Methods: Acetylphenylhydrazine was used to establish the rat model of erythronoclastic anemia and after a week the rats except control and model groups were given to Lvjiao Buxue Granules (8 g/kg) separately once daily for two weeks. While the rats in the control and model groups were ig given equivalent distilled water respectively. The endogenous metabolites in serum from all of rats were analyzed by 1H-NMR coupled with multivariate statistical analysis. Results: Compared with the control group, the levels of lipids, lactic acid, and acetone in serum of rats in the model group increased while the levels of alanine, valine, creatinine, phosphocholine, glycerophosphocholine, oxidation of three methyl ammonium, glycine, and arginine were decreased. The levels of these endogenous metabolites with significant difference were reversed to normal by ig asminstration of Lvjiao Buxue Granules. Conclusion: The mechanism of Lvjiao Buxue Granules on tonifying blood is involved in the pathway of energy metabolomic, lipid metabolism, and intestinal bacteria metabolism.