Carbazole alkaloids from Clausena excavata and their antitumor activity / 中草药

ABSTRACT

Objective: To study the chemical constituents from the roots of Clausena excavata (Rutaceae). Methods: The compounds were isolated and purified by means of column chromatographies including silica gel, RP-18, and HPLC. Compound structures isolated were determined on the basis of spectroscopic data. Results: Thirteen carbazole alkaloids clausine K (1), clausine E (2), 3-formyl-7-hydroxycarbazole (3), clausine H (4), clausine M (5), mukona (6), O-demethylmurrayanine (7), clausine O (8), 3-formylcarbazole (9), 3-formyl-6-methoxy carbazole alkaloid (10), clausine C (11), 2-methoxy-3-carboxylic acid-carbazole alkaloid (12), and clausine Z (13) were isolated from the roots of C. excavata. Conclusion: Compounds 9,10, and 12 are isolated from this plant for the first time. Compounds 1,2,7,8, and 10 are tested for their cytotoxicities against A549, Hela, and BGC-823 cancer cell lines. The results show that they exhibit the cytotoxicity against A549, Hela, and BGC-823 with the IC50 values of 8.53-19.87 μg/mL.