Preparation and characterization of epigallocatechin-3-gallate chitosan nanoparticles / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 741-747, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-853668
ABSTRACT
Objective:
To prepare the epigallocatechin-3-gallate (EGCG) chitosan nanoparticles (CS-NPs) and investigate their physicochemical properties.Methods:
The EGCG-CS-NPs were prepared by ion gelation method. The formulation variables were optimized by Box-Behnken Design (BBD) of response surface methodology (RSM) of CS concentration (X1), sodium tripolyphosphate concentration (X2), and EGCG concentration (X3) as independent variables and encapsulation efficiency (Y1,%) and particle size (Y2, nm) as dependent variables. The optimized CS-NPs were characterized for encapsulation efficiency (EE), particle size, Zeta potential, morphology, and in vitro drug release behavior of EGCG-CS-NPs were studied.Results:
An optimal EGCG-CS-NPs consisting of CS concentration as 2.6 g/L, sodium tripolyphosphate concentration as 1.5 g/L and EGCG concentration as 2.7 g/L. For EE, particle size, Zeta potential of EGCG-CS-NPs were found to be (85.8±3.1)%, (102.2±27.1) nm, and (25.5±4.1) mV, respectively. The CS-NPs were found to be small and spherical as seen in transmission electron microscopy (TEM). The in vitro release data proved that the drug release was steady within 24 h (pH 4.5 PBS).Conclusion:
Through optimizing the formulation, we obtain the uniform EGCG-CS-NPs with in vitro sustained-release behavior. This work is useful for the further research on pharmacodynamics of EGCG-CS-NPs.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2016
Tipo de documento:
Artigo
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