A new isocoumarin isolated from endophytic fungus Arthrinium sp. A092 derived from medicinal plant Uvaria microcarpa / 中草药

ABSTRACT

Objective: To study the chemical constituents in fermented mycelium of endophytic fungus Arthrinium sp. A092 from Uvaria microcarpa. Methods: The compounds were isolated and purified by chromatography on silica gel, Sephadex-LH20 columns, HPLC, and so on. Their structures were identified on the basis of physicochemical properties and spectroscopic data. All compounds were tested for their cytotoxic activities against four tumor cell lines HepG-2, MCF-7, NCI-H460, and SF-268. Results: Ten compounds were isolated from the mycelial extract and identified as 3,4,5-trimethyl-6-methoxy-8-hydroxyisocoumarin (1), decarboxycitrinone (2), 4-hydroxy-17R-methylincisterol (3), 4-hydroxy-3-methoxybenzoic acid (4), dibutyl phthalate (5), flemingipanic acid (6), indole- 3-carboxy acid (7), ergosterol peroxide (8), p-hydroxybenzoic acid (9), and 4-hydroxybenzal-dehyde (10). Compounds 3 exhibited the inhibitory activity against SF-268 and MCF-7 with IC50 values of 63.8 and 57.2 μmol/L, respectively. Compounds 8 exhibited the inhibitory activity against SF-268, MCF-7, and NCI-H460 with IC50 values of 50.6, 32.3, and 39.0 μmol/L, respectively. Conclusion: Compound 1 is a new compound named arthrinisocoumarin A. Compounds 3-5 and 7-10 are isolated from the fungus of Arthrinium sp. for the first time. Compounds 3 and 8 show the moderated cytotoxic activities against SF-268, MCF-7, and NCI-H460.