Major phenolic acids in Usneae Filum by UHPLC-Triple-TOF-MS / 中草药

ABSTRACT

Objective: To establish a quick method of ultra-performance liquid chromatography-quadrupole time-of-fight mass spectrometry (UHPLC-Triple-TOF-MS) for the identification of phenolic acids (diffractaic acid, usnic acid, orsellinic acid, etc.) in Usneae Filum. Methods: The separation was performed on the chromatographic column of Phenomenex Luna 3u C18 (150 mm × 2.0 mm, 3 μm), and the mobile phase was methanol (0.05% formic acid)-0.05% formic acid solution (4 mmol ammonium acetate), with a gradient elution at a flow rate of 0.3 mL/min. The column temperature was 30℃, and negative ion mode was used for TOF-MS. Results: Seventeen compounds were identified or tentatively characterized based on the retention time and MS spectra. They are depsides, two benzo furan, ant multi substituted single benzene as well. And the preliminary fragmentation rules are summarized. Conclusion: The results demonstrate that UHPLC-Triple-TOF-MS method is novel, quick, and efficient for the identification of the compounds in Usneae Filum.