Spectrum-effect relationship of active fraction from Hedysari Radix on improving immunity / 中草药

ABSTRACT

Objective: To study the correlation of HPLC fingerprint of Hedysari Radix and efficacy of improving immunity of normal mice, and provide the basis for finding bioactive components and selecting the index composition of quality control. Methods: The mice delayed type hypersensitivity (DTH) model induced by dinitoflruorobenzene (DNFB) was used to study the effect and then to perform ig administration with different extracts of Hedysari Radix for selecting the best part. To study the correlation between fingerprint and efficacy and find out the effective substances foundation of improving immunity, the peak area of each common peak from HPLC fingerprint was associated with the date of improving immunity in the method of partial least square regression (PLS). Results: In ear swelling experiment, water extract of Hedysari Radix was the most effective part which could improve the immunity function. PLS was used to evaluate the correlation of effect of improving immunity and components of the water extract including adenosine, ononin, genistein, formononeti, and medicarpin, the index of compounds to characterize the quality of Hedysari Radix, showed the negative correlation with the effectiveness. Conclusion: A new method is established to evaluate the activity of enhancing immunity of Hedysari Radix. It is of practical significance as an effective approach for controlling quality and exploring the material basis for efficacy of traditional Chinese medicine compounds.