Analysis on Peucedani Radix coumarin by UPLC/Q-TOF MS and study on its preliminary pharmacodynamics / 中草药

ABSTRACT

Objective: To establish a UPLC/Q-TOF MS method for analyszing the chemical constituents of total coumarins extract from Peucedani Radix (the rhizome of Peucedanum praeruptorum) and to study its preliminary pharmacodynamics. Methods: The chromatographic column was Acquity UPLC BEH C18, the mobile phase was composed of 0.1% formic acid (A) and acetonitrile (B) with gradient elution; The detection wavelength was set at 321 nm; The ionization mode was ESI+; And the mass scan range was m/z 100-1 000. Using the rat model of type 2 diabetes mellitus (T2DM) induced by streptozocin (STZ), the effect of total coumarins on reducing blood-lipid and blood-sugar was investigated in the experiment. Results: Qianhucoumarin D, peucedanocoumarin II, praeruptorin A, peucedanocoumarin I, praeruptorin B, and praeuptorin E in Peucedani Radix coumarin could be well separated and identified in one run. The preliminary pharmacodynamic results show that the total coumarins had an effect of reducing the level of fasting blood glucose, cholesterol, triglycerides, LDL, and liver index (P < 0.05); On the other hand, it could improve the level of HDL (P < 0.05). The above results prompt that coumarin extract of Peucedani Radix could improve the carbohydrate and lipid metabolism of T2DM, then play a role in the treatment of T2DM. Conclusion: A UPLC/Q-TOF MS method has been established to separate and identify the total coumarin extract of Peucedani Radix, the preliminary pharmacodynamic study shows that the total coumarin extract of Peucedani Radix had the function of reducing blood sugar and blood lipid significantly.