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Study on antidiabetic effect of aqueous extract from Pulsatilla chinensis / 中草药
Chinese Traditional and Herbal Drugs ; (24): 3544-3548, 2015.
Artigo em Chinês | WPRIM | ID: wpr-853844
ABSTRACT

Objective:

To study the blood glucose lowering activity of aqueous extract from Pulsatilla chinensis (AEPC) on Streptozocin (STZ)-induced type-2 diabetic rats.

Methods:

AEPC was obtained through traditional aqueous extraction procedure; Oral glucose tolerance test was employed for the preliminary study of AEPC's blood glucose lowering activity; For further study, STZ-induced neonatal diabetic rat model was used, and the serum SGPT, SGOT, ALP, serum urea, triglycerides, total cholesterol, HDLC, total protein, and serum insulin were tested; The Kunming rats were used for acute toxicity experiments, with the highest dose of 1 000 mg/kg body weight; Insulin release from RIN 5F cells was performed to study the mechanism of AEPC on antidiabetes effect.

Results:

Oral glucose tolerance test showed AEPC could lower the blood sugar, and the optimal activity was observed at a dose of 20 mg/kg body weight; In neonatal STZ-induced type-2 diabetic rats, treatement with 20 mg/kg AEPC for 20 d resulted in significant blood glucose lowering activity, liver glycogen and serum biological parameters back to normal; The acute toxicity results showed that no obvious toxicity was observed even at 1 000 mg/kg dose; At last, AEPC could promote the secretion of insulin from RIN 5F cells.

Conclusion:

AEPC shows the anti-diabetes activity on STZ-induced type-2 diabetic rats, which may be the result of its effect on promoting insulin release from pancreatic α-cells.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional and Herbal Drugs Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional and Herbal Drugs Ano de publicação: 2015 Tipo de documento: Artigo