Genetic diversity analysis on Angelica sinensis from different habitats in Gansu province based on ISSR / 中草药

ABSTRACT

Objective: To investigate the genetic diversity of Angelica sinensis from different habitats in Gansu province. Methods: ISSR markers were used to analyze the genetic diversity of 41 populations of A. sinensis. Nei's genetic diversity index (H) and other parameters of genetic information were calculated by Popgene 32. UPGMA dendrogram relationship was clustered by Ntsys. Results: Eight primers produced 154 bands, among which 119 were polymorphic bands, the average percentage of polymorphie bands (PPB) was 77.27%. H and Shannon's information index (I) were 0.222 9 and 0.337 4, the genetic differentiation coefficient (Gst) and gene flow (Nm) were 0.6839 and 0.231 1 within the population levels. The genetic distance varied from 0.042 9 to 0.327 8. Conclusion: The genetic diversity among species of A. sinensis is at higher level, but the level of genetic diversity between populations is higher than that within populations. There is a great degree of genetic differentiation among populations, and gene flow does not almost exist between populations.