Simultaneous pharmacokinetics of rhein in rat plasma and kidney / 中草药

ABSTRACT

Objective: To investigate the pharmacokinetics of rhein (RH) in rat plasma and kidney simultaneously. Methods: An HPLC method was developed to determine the concentration of RH in rat plasma, which was corrected with protein binding. Renal microdialysis (MD) technique was employed to collect dialysate sample continuously. Then the MD samples were detected by HPLC-MS/MS method. Results: RH was distributed into kidney of rats rapidly after administration. The maximum concentration of RH in rat kidney was reached at 1 h followed by continuously decreased. During the whole process, the concentration of RH in rat kidney was significantly lower than that in plasma after administration. The MRT of RH in plasma and kidney was (87.217 ± 29.889) and (122.387 ± 12.521) min, respectively. The areas under the curve (AUC) of RH in plasma and kidney were (114.236 ± 45.585) and (16.596 ± 1.732) μg·min/mL, respectively. The kidney penetration was 0.161 ± 0.056. Conclusion: Simultaneous pharmacokinetic study in rat plasma and kidney is an effective method to investigate drug kidney penetration. RH can penetrate rat's kidney after administration to exert its effect.