In vivo pharmacokinetic study on several active components of Dachengqi Decoction in rats by UPLC-MS/MS / 中草药

ABSTRACT

Objective: To study the in vivo pharmacokinetics of aloe emodin, rhein, emodin, chrysophanol, physcion, hesperidin, neohesperidin, naringin, honokiol, and magnolol in plasma of rats after ig administration of Dachengqi Decoction. Methods: After ig administration of Dachengqi Decoction (10.35 g/kg), the blood was collected from the retinal vein plexus of rats at predetermined time and pre-prepared with 1,8-dihydroxyanthraquinone as internal standard (IS). A reliable ESI source, negative ion mode scanning, and multiple reaction monitoring (MRM) method were developed for the simultaneous determination of the active components in Dachengqi Decoction and chromatography was carried out on a Shimadzu Shim-pack XR-ODS III (75 mm × 2.0 mm, 1.6 μm) using a gradient mobile phase consisted of methanol and 0.1% formic acid water at a flow rate of 0.35 mL/min. Calculations of the pharmacokinetic data were performed using WinNonlin 4.1 software. Results: Calibration curves for aloe emodin, rhein, emodin, chrysophanol, physcion, hesperidin, neohesperidin, naringin, honokiol, and magnolol in plasma of rats were linear within the ranges of 0.52—520.00, 2.06—616.00, 0.57—568.00, 3.1—1 240.0, 18.5—1 000.0, 1.94—972.00, 1.59—796.00, 1.63—816.00, 0.025—50.000, and 0.027—53.300 ng/mL, respectively. The precision, recovery, and stability of each component in the present assay were acceptable for the analysis. They are in the line with the requirements of biological sample analysis. The concentration of physcion in plasma of rats was under the limit of detection at almost every time point, thus we failed to obtain the pharmacokinetic parameters of it. While the plasma concentration and pharmacokinetic parameters of other components were successfully calculated. Conclusion: The proposed method can be used for the simultaneous determination of the concentration of several active components in plasma of rats and successfully applied to the pharmacokinetic study of the analytes in rats after ig administration of Dachengqi Decoction.