Rapid propagation of Paris polyphylla var. chinensis / 中草药

ABSTRACT

Objective: The roots of Paris polyphylla var. chinensis were used as explants to establish a rapid propagation technique. Methods: The effects of hormone 6-BA, 2,4-D, NAA, IBA, KT, IAA, ceftriaxone, activated carbon, etc on callus induction, differentiation of adventitious bud, and formation of adventitious roots were studied to find out the best culture medium formulation by using MS as basic culture medium. Results: The best medium for callus induction of P. polyphylla var. chinensis was MS + 6-BA 1.0 mg/L + NAA 1.5 mg/L + 2,4-D 3.0 mg/L, and the best medium for differentiation of adventitious bud was MS + 6-BA 3.0 mg/L + IAA 0.3 mg/L + KT 1.0 mg/L + ceftriaxone 300 mg/L, while the best medium for formation of adventitious roots was 1/2 MS + IBA 0.5 mg/L + NAA 0.1 mg/L + activated carbon 0.5%. The tissue cultured seedling was moved to sterile water for 5 d when the first leaf emerged and four adventitious roots appeared, then the seedling was transplanted into the soft soil. The tissue cultured seedling would grow strongly with 100% survival rate. Conclusion: The adventitious bud is inducted and then the root from bare-root seedling is cultured on the basis of obtained callus. Finally the best conditions to foster the tissue cultured seedling of P. polyphylla var. chinensis have been screened.