Nine components in Shiwei Penan Granule and their fingerprint chromatography / 中草药

ABSTRACT

Objective: To determine the nine components in Shiwei Penan Granule and establish the fingerprnt analysis for the quality control of Shiwei Penan Granule. Methods: The method was performed on a Thermo C18 column (250 mm × 4.6 mm, 5 μm); The gradient mobile phase consisted of acetonitrile (A)-0.1% phosphoric acid (B) with a flow rate of 1.0 mL/min (0-16 min, 5%-14% A; 16-20 min, 14%-17% A, 20-35 min, 17%-19% A; 35-60 min, 19%-35% A; 60-62 min, 35%-75% A; 62-78 min, 75% A); The detection wavelength was set at 230 nm (for quantitative analysis and fingerprint). Results: Gallic acid, sodium danshensu, chlorogenic acid, paeoniflorin, polydatin, benzoic acid, salvianolic acid B, emodin, and physcion were baseline seperated with good linearity relationships (r > 0.999 9) between concentration and peak areas over the linear ranges. The average recoveries of the compounds were 100.15% (RSD = 1.39%), 99.89% (RSD = 1.71%), 99.92% (RSD = 0.67%), 99.28% (RSD = 0.60%), 99.89% (RSD = 0.80%), 99.72% (RSD = 1.83%), 99.91% (RSD = 0.79%), 100.06% (RSD = 0.94%), and 99.97% (RSD = 1.36%). Using Traditional Chinese Medicine Fingerprint Similarity Evaluation System (2012 Edition) to analyze the fingerprint of 15 batches of Shiwei Penan Granule, the similarity values between the reference fingerprint and the 15 batches were higher than 0.977. Conclusion: The method is simple, rapid, and accurate, and can be used as an effective method to evaluate the quality of Shiwei Penan Granule.