Methodological study on quality evaluation of crude and processed Pinelliae Rhizoma based on antitussive bioassay / 中草药

ABSTRACT

Objective: To establish a method for determining the antitussive effects of Pinelliae Rhizoma (Pinelliae ternate), Pinelliae Rhizoma Praeparatum cum Alumine, Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine, Pinelliae Rhizoma Praeparatum and Pinelliae Rhizoma Praeparatum cum Glycyrrhizae et Alumine, and provide a scientific basis for constructing a new quality evaluation pattern of crude and processed P. ternate. Methods: Antitussive bioassay for crude and processed Pinelliae Rhizoma was established by taking parallel line quantitative analysis method, based on mouse cough model induced by strong aqua ammoniac. Correlation analysis was used to determine the relationships among the levels of different total organic acids and antitussive bioassay. Results: The results showed that antitussive bioassay increased after prepared. Pinelliae Rhizoma Praeparatum cum Glycyrrhizae et Alumine had the highest antitussive bioassay (2105.59 U/g), followed by Pinelliae Rhizoma Praeparatum, Pinelliae Rhizoma Praeparatum cum Alumine and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine; Pinelliae Rhizoma demonstrated the lowest antitussive bioassay which was only 1/3 of Pinelliae Rhizoma Praeparatum cum Glycyrrhizae et Alumine. The antitussive bioassay increased when the content of total organic acids was growing, presenting a significant positive correlation, R2 among them ranged from 0.6920 to 0.9178. Conclusion: The method is stable, precise, and good for determining the antitussive bioassay of crude and processed Pinelliae Rhizoma. It can be used in quality evaluation and quality standard and provide a scientific basis for the quality control of Chinese materia medica.