Distribution in mouse tissues of P-80 modified neurotoxin nanoparticles after intranasal administration in rats / 中草药

ABSTRACT

Objective: To prepare neurotoxin nanoparticles (NT-NP) labeled by FITC and modified by polysorbate-80 (P-80), and to compare the in vivo tissue distribution after intranasal administration in rat. Methods: The FITC labeled P-80-NT-NP and NT-NP were prepared, blood samples were collected after intranasal administration in rats at 5, 15, 30, 60, 120, and 240 min, and the brain, heart, liver, spleen, lung, kidney tissue samples were collected. Using FITC-NT as the index component and fluorescence analysis method, the differences of the nanoparticles distribution in rats tissues after modified by P-80 were investigated. Results: After 5, 15, 30, 60, 120, and 240 min of intranasal administration of P-80-NT-NP and NT-NP, the drug was distributed in plasma, heart, liver, spleen, lung, kidney, and brain. After 120 min of administration, the NT concentration in plasma and brain of P-80-NT-NP group was higher than that of NT-NP group, while the NT concentration in liver was lower than that in NT-NP group, with significant difference. Conclusion: P-80 can effectively increase the nasal absorption of NT-NP into the brain and other tissues, which provides the basis for further development and application of preparation of the drug targeting to brain.