Resource components analysis on Salvia miltiorrhiza residues in production process of Danhong Injection and its transformation mechanism / 中草药

ABSTRACT

Objective: To analyze the resource components (tanshinones and salvianolic acids) in Salvia miltiorrhiza residues in production process of Danhong Injection, explore the constituents transformation mechanism, and discover the resource utilization pathway for S. miltiorrhiza residues. Methods: The chemical constituents in S. miltiorrhiza and S. miltiorrhiza residues were extracted by methanol ultrasonic method. The HPLC-PDA analysis was carried out on a BDS HYPERSIL C18 (250 mm×4.6 mm, 5 μm) column with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution. The flow rate was 1.0 mL/min, the column temperature was maintained at 30℃. Then the residues resource utilization was further explored based on the research results and previous work. Results: The tanshinone components were virtually unused and remained in the residues during the production process of Danhong Injection. The contents of salvianolic acid A and protocatechuic aldehyde in the residues are higher than those in the salvia herbs. Conclusion: The tanshinones in S. miltiorrhiza residues are worthy of further development and utilization during the Danhong Injection production process. It provides a main basis for the resource utilization and development of S. miltiorrhiza residues.