Chemical constituents of Epimedium brevicornu / 中草药

ABSTRACT

Objective: To study the chemical constituents of Epimedium brevicornu. Methods: Compounds were repeatedly separated and purified with D101 macroporous resin column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography and preparative liquid chromatography. Their structures were identified by physico-chemical methods and spectral methods. Results: Six xompunds were identified as 5-hydroxy-2-prenylphenol-1-O-β-D-glucopyranoside (1), kaempferol-3-O- rhamnopranosyl-(1→2)-rhamnopyranoside (2), 3-O-α-L-rhamnosyl-7-O-β-D-glucosyl kaempferol (3), sagittasine C (4), kaempferol-3- O-[α-L-rhamnopyra-nosyl-(1→6)-β-D-galactopyranoside]-7-O-α-L-rhamnopyranoside (5), 3,7-di-O-α-L-rhamnopyranosyl quercetin (6), Kaempferol-3,7-O-α-L-dirhamnoside (7), hamnocitrin-3-O-β-neohesperidoside (8), kaempferol-3-O-α-L-rhamnopyranoside-7- O-[α-L-rhamnopyranosyl (1→2)-β-D-glucopyranoside] (9), hexandraside E (10), and rouhuoside (11). Conclusion: Compounds 1 and 5 are isolated from this plant for the first time. Compounds 2-4 and 6 are isolated from plants of this genus for the first time.