Simultaneous determination of eight active components in Gastrodiae Rhizoma by HPLC-MS / 中草药

ABSTRACT

Objective: To establish an HPLC method for the simultaneous determination of eight active components (gastrodin, 4-hydroxybenzyl alcohol, vanillyl alcohol, vanillin, p-hydroxybenzaldehyde, parishin B, parishin C, and parishin A). Methods: The analysis was performed on an Agilent 1220 system with a Hypersil C18 column (250 mm × 4.6 mm, 5 μm). The eight components were separated in 75 min with gradient mobile phase consisting of 0.5% CH3COOH-H2O (A) and 0.5% CH3COOH-CH3OH (B) 0-10 min, 98% A; 10-60 min, 98%-60% A; 60-75 min, 60% A. The temperature of column was 30 ℃, and the injection volume was 20 μL. The multiple-reaction monitoring (MRM) scanning was employed for the quantification with switching electrospray ion source polarity in negative mode. The ion spray voltage was set at -4500 V and the turbo spray temperature was maintained at 550 ℃. Results: The eight components had the good linearity (r ≥ 0.999 2) within the linear ranges. The average recovery rate was 94.51%-102.70% with RSD < 3.50%. The contents of the eight components of Gastrodiae Rhizoma varied according to the different habits. The contents of parishins A, B, and C were high while the contents of vanillyl alcohol and vanillin were low. Conclusion: The developed HPLC-MS method is simple, sensitive, and accurate, and has the good repeatability in separation, which is available for the quality control of Gastrodiae Rhizoma.