Effect of Angelica sinensis polysaccharide on Wnt/β-catenin signaling pathway in hematopoietic stem cells of aging model mice / 中草药

ABSTRACT

Objective: To explore the effects of Angelica sinensis polysaccharide (ASP) on Wnt/β-catenin signaling pathway in hematopoietic stem cell (HSC) of aging model mice Methods: Male Thirty C57BL/6J mice aging 6-8 weeks were randomly divided into normal group, aging model group, and ASP aging model group (ten in each group). After 2 d of finishing the treatment, magnetic activated cell sorting (MACS) were applied to the HSC from mouse bone marrow respectively. The ratio of the SA-β-Gal staining positive HSCs were counted; The capability of colony formation was examined by CFU-Mix cultivation; The distribution of ROS levels was analyzed by flow cytometry (FCM) and laser scanning confocal microscope assess; The content of advanced glycosylation end products (AGEs) was detected by Elisa; The proteins of β-catenin in cytoplasm, GSK-3β in nucleus, Phospho-GSK-3β, and TCF-4 were detected by Western blotting. Results: Compared with the normal group, the percentage of SA-β-Gal, the product of ROS positive cells and AGEs in the aging model group were significantly increased; The expression of β-catenin in cytoplasm, β-catenin in nucleus, Phospho-GSK-3β, and TCF-4 were evidently up-regulated; The colony formation of CFU-Mix was markedly decreased; The expression of GSK-3β was evidently down-regulated. Compared with the aging model group, in ASP aging model group, the percentage of SA-β-Gal, the product of ROS positive cells, and AGEs were significantly decreased; The expression of β-catenin in cytoplasm and nucleus, Phospho-GSK-3β, and TCF-4 were evidently down-regulated; The colony formation of CFU-Mix was markedly increased; The expression of GSK-3β was evidently up-regulated. Conclusion: ASP can protect HSC from aging by antagonizing D-galactose, and the mechanism may be ASP inhibiting the excessive activation of Wnt/β-catenin signaling pathway.