Effects of 10 furanocoumarins in Angelica dahurica on intestinal transport of vincristine / 中草药

ABSTRACT

Objective: To study the effects of 10 kinds of furanocoumarines in Angelica dahurica (oxypeucedanin, isoimperatorin, phellopyerin, isoimperatorin, imperatorin, byakangelicol, angelicin, scopoletin, umbelliferone, and byakangelicine) on the intestinal absorption and transportation of vincristine. Methods: The apparent permeability coefficient (Papp) of vincristin was used as evaluated parameter, and Caco-2 (human colon cancer cell lines) cell monolayer was used as intestinal epithelial cell model to study the effects of 10 furanocoumarins on transportation of vincristine in Caco-2 cell monolayer. Results: There were different effects of the 10 furanocoumarins on transportation of vincristine. Oxypeucedanin, isoimperatorin, and imperatorin could improve the transport of vincristine in Caco-2 cell monolayer (P < 0.05), while isoimperatorin, byakangelicol, angelicin, umbelliferone, and byakangelicine could inhibit the transport of vincristine in Caco-2 cell monolayer (P < 0.01). However, phellopyerin and scopoletin have no effects on the transport of vincristine on Caco-2 cell. Conclusion: Different kinds of furanocoumarins have different effects on intestinal transport of vincristine, which may be related to the structure of furocoumarins and the functional group about their connection.