Study on inclusion process of decomposed volatile oil from Atractylodes macrocephala with hydroxypropyl-β-cyclodextrin / 中草药

ABSTRACT

Objective: To optimize the inclusion process of decomposed volatile oil from Atractylodes macrocephala (DVOA) with hydroxypropyl-β-cyclodextrin (HP-β-CD). Methods: The inclusion complex was prepared by the freeze drying method. The preparation process was evaluated using fluorescence spectrophotometry. The inclusion process conditions were screened by the orthogonal test, with the inclusion ratio and oil ratio as indexes, and mass ratio between HP-β-CD and DVOA, inclusion temperature and inclusion time as the impacting factors. The inclusion complex was characterized through ultraviolet-visible spectra (UV-vis), fluorescence spectra (FL) and microscopic imaging, and the stability test was performed. Simultaneously, the chemical composition in DVOA before and after inclusion was analyzed by GC-MS technique. Results: The DVOA and HP-β-CD had formed inclusion complex, the optimal inclusion conditions were as follows The mass ratio of HP-β-CD to DVOA was 101, the inclusion temperature was 30℃, the inclusion time was 2.0 h, and the ratios of average inclusion and oil were 73.32% and 10.43%, respectively. The chemical composition of DVOA was consistent before and after inclusion, the inclusion just had the slight effects on proportion of each component. Conclusion: The preparation process of DVOA/HP-β-CD inclusion complex under the optimal conditions is reasonable, stable, and feasible, and can provide the reliable experimental basis for the anti-tumor new drug research and development of DVOA.