Multi-indicators rapid determination in alcohol precipitation of Lonicerae Japonicae Flos and Artemisiae Annuae Herba in Reduning Injection by near-infrared spectroscopy / 中草药

ABSTRACT

Objective: Near-infrared (NIR) spectroscopy was used to rapidly determine the concentration of chlorogenic acid (CA) and soluble solid content in alcohol precipitation of Lonicerae Japonicae Flos and Artemisiae Annuae Herba (LA) in Reduning Injection, so as to increase the level of quality control of alcohol precipitation process. Methods: The LA samples of alcohol precipitation were collected. The PLS algorithm was used to establish NIR models between spectra and concentration of CA and total solid content, and the concentration of CA and soluble solid content in unknown batch were predicted. Results: The correlation coefficients (R) of NIR models were above 0.980 in calibration sets. The R of verification model was above 0.980, the root mean square error of prediction (RMSEP) for CA and soluble solid content were 0.541 and 0.218, respectively, and the relative standard errors (RSEP) for CA and soluble solid content were no more than 8%. Conclusion: The results demonstrate that NIR could determine the concentration of CA and soluble solid content in alcohol precipitation of Reduning Injection rapidly and accurately.