Identification of DNA barcoding on medicinal plants in Flemingia Roxb. ex Ait. et Ait. f / 中草药

ABSTRACT

Objective: To screen a DNA barcoding sequence that can identify the medicinal plants of Flemingia Roxb. ex Ait. et Ait. f. accurately and efficiently. Methods: Four species and 14 individuals of Flemingia Roxb. ex Ait. et Ait. f. were collected, the (second internal transcribed spacer, ITS2) of ribosomal DNA, rbcL, psbA-trnH of chloroplast DNA were amplified and sequenced. Meanwhile, the NCBI data were retrieved and the according sequence was downloaded. The total numbers of species and individuals were six and twenty. The PCR amplification and sequencing efficiency, barcoding gap, and NJ trees were used to evaluate the efficiency of species identification. Results: The sequencing success rates of ITS2, rbcL, and psbA-trnH were 100%, 100%, and 85.71%, respectively; Among the three DNA barcoding sequences, only ITS2 has remarkable barcoding gap; ITS2 could distinguish every species of Flemingia Roxb. ex Ait. et Ait. f. (except F. philippinensis and F. stricta). Conclusion: ITS2 could identify the medicinal plants of Flemingia Roxb. ex Ait. et Ait. f. accurately and efficiently, and could be used as an ideal DNA barcoding of species identification for medicinal materials of Flemingia Roxb. ex Ait. et Ait. f.