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Directional separation of active ingredients of dehydrotumulosic acid from Guizhi Fuling Capsule by molecular imprinting technique / 中草药
Chinese Traditional and Herbal Drugs ; (24): 853-856, 2015.
Artigo em Chinês | WPRIM | ID: wpr-854222
ABSTRACT
To establish a method for directional separation of dehydrotumulosic acid from the extracts of Guizhi Fuling Capsule with molecular imprinting technique (MIT). Molecular imprinting polymer (MIP) was prepared by sol-gel process with dehydrotumulosic acid as molecular template to study the absorption property. The dehydrotumulosic acid was achieved from Guizhi Fuling Capsule by one-step separation with polymer as filler. The structure of dehydrotumulosic acid was identified on the basis of the spectral data and physicochemical property. The maximum binding capacity (Qmax) of MIP was 9.10 mg/g measured by Scatchard equation and the purity of dehydrotumulosic acid was 90.76% by HPLC. The established MIT for the directional separation of dehydrotumulosic acid from Guizhi Fuling Capsule is simple and benefit to reducing the solvent use during the separation process, which could offer a novel method for the separation and purification of dehydrotumulosic acid.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional and Herbal Drugs Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Chinese Traditional and Herbal Drugs Ano de publicação: 2015 Tipo de documento: Artigo